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Proteins were separated on 12% gels and then blotted on a nitrocellulose membrane in a wet blotting chamber at 4C, using 250 mA per gel

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8 A melanotic macule, by contrast, is typically well-defined, uniform in color, stable in size, and flat

GIP (glucose-dependent insulinotropic polypeptide) is another gut hormone

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Therefore, Prevotella copri and Mitsuokella may be related to pathogenesis of T2D and its complications