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Simultaneous quantification of these two oxidized metabolites by electrochemical HPLC assay demonstrated that human P450 2E1 selectively oxidizes APAP to NAPQI (determined as glutathione conjugate, GS-APAP), whereas human P450 2A6 selectively oxidizes APAP to 3-OH-APAP (Chen et al., 1998)

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Finally, the samples were prepared for Western blotting analysis by lysing the brain homogenates on ice for 300 minutes in 100 mL of a lysis buffer composed of 120 mM NaCL, 40 mM Tris (pH 8), and 0.1% NP40, followed by centrifugation

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