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Optimization of Soluble Expression and Purification of Recombinant Human Rhinovirus Type-14 3C Protease Using Statistically Designed Experiments: Isolation and Characterization of the Enzyme

Participants were randomized to a control group (n = 40), receiving standard Western medical treatment, or an experimental group (n = 40), receiving electroacupuncture at selected alternative acupoints in addition to standard care

DOI: 10.1038/s41398-020-01135-3

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In vitro assays with 3,4- cis -leucocyanidin (6) Standard reaction conditions for in vitro one-step and two-step biotransformations of 3,4- cis -leucocyanidin ( 6 ) were 100 mM MOPSO, pH 6.2, 20 mM sodium ascorbate, 1 mM -ketoglutaric acid, 0.4 mM ammonium iron(II) sulfate, 200 M 3,4- cis -leucocyanidin ( 6 ) and 20 M GSH

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