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Allergic reactions possible in sensitive individuals though rare

Patients should select a specific day of the week and time of day for their injection, using calendar reminders, smartphone alarms, or medication tracking apps to ensure they do not miss doses

After the corresponding treatment, cells were fixed with cold methanol for 5 min, blocked with 1% BSA in phosphate-buffered solution (PBS) for 1 h, and then incubated overnight at 4 C with anti-collagen 1 type I antibody (Santa Cruz), anti-collagen IV (20421, Novotec, Lyon, France), collagen 1 type III (sc271249, Santa Cruz), anti-fibronectin (sc8422, Santa Cruz) and LOX (ab174316, Abcam), followed by the corresponding fluorescent secondary antibodies

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