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Animal experiments have demonstrated that high-fat diet downregulates tight junction protein expression (such as occludin and ZO-1), reduces mucus layer thickness, and increases intestinal permeability ( Metabolic endotoxemia refers to a state of chronically elevated circulating LPS levels caused by diet (particularly high-fat diet) (92) first proposed this concept, finding that high-fat diet mice exhibited significantly elevated plasma LPS levels accompanied by increased Gram-negative bacteria proportions and enhanced intestinal permeability
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The level of type III collagen, a marker of myocardial fibrosis, is significantly increased in an iron-overloaded rat model of type 1 diabetes mellitus [181]
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