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Nuclei were pelleted by centrifugation (650 g, 5 min), supernatant was discarded and the pellet was resuspended in 100 l NUN1 buffer containing 20 mM Tris-HCL (pH = 7.5), 75 mM NaCl, 0.5 mM EGTA, 50 % glycerol, 1x protease and 1x phosphatase inhibitors

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Knowing the difference can help you respond appropriately

Exogenous MOTS-c supplementation may partially restore this responsiveness

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These devices represent a leap forward from the traditional syringe-and-needle approach, providing a less invasive method of drug delivery