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Fluctuations in ROS levels can influence the function of protein kinases, phosphatases, and various enzymatic proteins, thereby affecting critical processes such as cellular metabolism, gene regulation, and proliferation [25]

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The time course of peptide degradation was determined by integrating the area of each peak in a series of HPLC traces

This is then determined using a high-performance liquid chromatograph equipped with a diode array detector or ultraviolet detector, with quantification performed by the external standard method