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Cysteine, unlike most other amino acids, is extremely unstable and rapidly autoxidizes to cystine which is the oxidized disulphide form

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Moreover, interspecies differences in ovarian tissue architecture, mitochondrial density, and endogenous antioxidant capacity can lead to variable outcomes, with certain antioxidants demonstrating stronger protective effects in rodent models compared to human or ruminant tissues (93)

8.05 10 7 cells per replicate were then passaged without puromycin on days 6 and 10

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